Fig. S5
Some integrins are not regulated by snai1b. (A-H): In situ hybridization for indicated genes. (A-D): β1 integrin expression was not changed by snai1b knockdown at 15 hpf. (A, C): dorsal view, (B, D): lateral view. (E-H): β3a and β3b integrin expression was not detected at 15 hpf (E, F), but at 72 hpf (G, H). (I): qRT-PCR showed that α8 and αV integrin transcription was not regulated by snai1b at 15 hpf. (J): qRT-PCR showed that α5 integrin transcription was up-regulated by snai1b overexpression, but not β1 integrin transcription at 15 hpf. The increased of α5 integrin transcription was statistically significant (P=0.007, t-test).