ZFIN Image: Coxam et al., 2014, Fig. S2

Image

Figure Caption

Fig. S2

Parachordal lymphangioblasts fail to directionally migrate and display altered cell dynamics in the lyc1 mutant. Related to Figure 1.

(A-B) Schematic overview of the movement of the leading front of individual parachordal lymphangioblasts (t=10.5h) from 56 hpf (scale bar: 10 μm) in (A) WT/MO-pkd1b (5ng MO) (n=3 embryos, n=4 leading fronts) and (B) phenotypically mutant lyc1/ MO-pkd1b (5ng MO) (n=5 embryos, n=5 leading fronts).

(C-D) Quantification of (C) movement of cell front and (D) origin to endpoint of cell front distance migrated by parachordal lymphangioblasts (t=10.5h) from 56 hpf in in WT/MO-pkd1b (5ng MO) (n=3 embryos, n=4 leading front) and phenotypically mutant lyc1/ MO-pkd1b (5ng MO) (n=5 embryos, n=5 leading fronts) (μm).

(E) Quantification of duration of individual filipodial extensions in single parachordal lymphangioblasts at the horizontal myoseptum by time-lapse imaging (spinning disc). WT/MO-pkd1b (n=3) and phenotypically mutant lyc1/MO-pkd1b embryos (n=4) (5ng MO) were examined between 56-64 hpf (1 time unit = 4 minutes).

(F) Quantification of the number of filipodial extensions per parachordal lymphangioblasts by time-lapse imaging (spinning disc) in WT/MO-pkd1b (n=3) and phenotypically mutant lyc1/MO-pkd1b embryos (n=4) (5ng MO) between 56-64 hpf.

Figure Data

Phenotype details

Acknowledgments

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