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Fig. 3
Knockdown of sox2 results in upregulation of neurogenesis.
(A–B) Neurogenesis is increased in sox2 morphants, as judged by Isl1 expression. (C) Average number of Isl1-positive cells in control (purple bar) and sox2 morphants (orange bar), at 24 and 28 hpf. (D–E) At 3–4 ss, two flh-domains are observed on either side of the neural plate. (F–G) By 7–8 ss, the two domains fuse to form the presumptive pineal gland. (H–K) In sox2 morphants, flh is expressed in broader domains in relation to control siblings. Scale bars = 25 μm, (A–B) confocal maximum projections, (C) error bars represent ± standard error, ** = p-value <0.001 (MWU test). (J,L,N,P) dorsal views with anterior to the top, (K,M,O,Q) frontal views. See also Figure S2–S3.