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Fig. 1
The zebrafish sox2 morphant model.
(A) Lateral view of control embryo at 52 hpf. (B) sox2 morphants have small eyes and short body axis in relation to control siblings. (C) Microinjections with SOX2 mRNA have no phenotypic effect, while (D) mRNA injections into sox2 morphants rescue the phenotypes. (E) Western blot showing the reduction in the levels of Sox2 in embryos injected with sox2-MO1 (second column) and sox2-MO2 (third column), when compared to controls (first column). β-tubulin was used as loading control. (F) Whole mount immunofluorescence for Sox2 (red) and Isl1 (blue) shows that Sox2 is expressed in the epithalamus, but not in the differentiated pineal cells at 28 hpf. (G) Sox2 is undetectable by immunofluorescence in sox2 morphants, at 28 hpf. (H–I) Morpholino microinjections do not grately affect apoptosis, as judged by TUNEL assay at 28 hpf. (A–D) scale bars = 250 μm, (F–G) scale bars = 25 μm. See also Figure S1.