|
Fig. 6
Genetic interaction between zbbs1 and znphp7.2 in vivo.
Injected zebrafish embryos were assessed for the incidence of pronephric cysts. (A) Cont MO injected embryos with normal pronephros. While the majority of zbbs1 or znphp7.2 morphants (suboptimal dose) showed pronephros of normal morphology, those of combined knockdown exhibited pronephros with cysts. Cysts are marked by asterisks. (Black scale bar = 500 μm, White scale bar = 100 μm) (B) Pronephric cysts were detectable after individual injections of znphp7.2 MO at 0.1 mM and zbbs1 MO at 0.2 mM with the level of 17% and 10% respectively whereas the combined knockdown caused pronephric cysts in 59% of microinjected embryos. The final MO concentration for injection was 0.3 mM in all conditions to keep total MO dose constant. Therefore suboptimal doses of zbbs1 and znphp7.2 MO were combined with Cont MO to obtain this final concentration of 0.3 mM. The numbers in the brackets (n) are the numbers of total embryos which were examined.