ZFIN Image: Acosta et al., 2014, Fig. 4

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Figure Caption

Fig. 4

Ubiquitous FUS-GFP SG assembly in zebrafish cells.

(A) FUS-GFP SGs formed in cultured transgenic zebrafish cells after heat-shock at 40°C for 30 mins (arrows). However, SGs were more abundant in mutant FUS-R521C-GFP cultures (right panels) than in FUS-WT-GFP. (B) Motor neurons (39.4D5-labeled cells) were not particularly susceptible to SG assembly. (C) SGs were reversible when cells were allowed to recover at 37°C for another 30 mins. Some persistent SGs were still present particularly in FUS-R521C-GFP cultures. Motor neurons labeled with 39.4D5 readily reversed SGs. (D) Stress granules (SGs) were also induced by sodium arsenite (Na₃AsO₃; 0.2 mM) treatment. SGs formed in mutant (arrows) but not in the FUS-WT-GFP line after Na₃AsO₃ treatment for 1 hr. Similar to heat-shocked cells, 39.4D5-labeled cells were no more susceptible to chemical-induced SG formation. (E) Chemical-induced FUS-GFP containing SGs were reversible in both lines. Reversibility also occurred readily in 39.4D5 labelled motor neurons.

Acknowledgments

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