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Fig. S12
Wnt signaling inhibition delays neutrophil resolution in amputated larval fins. (A) Representative images (24 hpa) of a loss-of-function Wnt/β-catenin signaling line Tg(hsDKK1:GFP) crossed to a Tg(lyzC:dsRed) line to detail a regenerative timecourse of neutrophil accumulation in larval fins near the injury site. Fins on wildtype (WT) or loss-of-function Wnt/β-catenin signaling fish (hsDKK) were resected, and heat shocked once daily (hsWT, hsDKK) for 80 hpa. (B) Quantification of neutrophil accumulation in injury area after fin amputation for hsDKK and WT fish indicating increased cell number in DKK1-overxpressing fins at time points from 16 hpa through 60 hpa. (C) Quantification of neutrophil accumulation in injury area after fin amputation in gain-of-function Wnt/β-catenin signaling line Tg(hsWnt8a:GFP) crossed to a Tg(lyzC:dsRed) line. Unlike loss-of-function Wnt/β- catenin signaling fish (hsDKK1), Wnt8a-overexpressing larvae show no significant difference in macrophage number with hsWT. Data are representative of three independent experiments with at least 6-8 fish per timepoint. Error bars represent the s.e.m. * vs hsWT; P(16 hpa)=0.0140, P(20 dpa) = 0.0125, P(28 dpa) = 0.0399.