|
Fig. 3
Leukocytes do not enter the notochord during the early phase of infection. (A,B) Multiscan confocal analysis at 2 hpi of neutrophils (green) in Tg(mpx:GFP) larvae after muscle (A) or notochord infection (B) with DsRed-expressing E. coli. (C,D) Multiscan confocal analysis of macrophages (red) in Tg(mpeg1:mCherryF) larvae after intravenous (C) or notochord (D) infection with E. coli-GFP (green) at 2 hpi. In A–D, the left panels are xy maximum projections and the right panels are yz cross-section views at the position of the stippled line. Arrowheads show bacteria-containing leukocytes, *Leukocytes that were located next to bacteria but did not contain any. (E–G) Confocal analysis at 24 hpi of the notochord with fluorescent E. coli. Bacteria were no longer visible at 24 hpi. (E,E2) Tg(mpx:GFP) larva showing neutrophils (green), (F,F2) Tg(mpeg1:mCherryF) showing macrophages, (E,F) maximum projection, (E2,F2) single confocal scan. (G) Confocal analysis of a 60-μm cross-section from a double-transgenic larva – neutrophils (green) and macrophages (red) accumulated around the notochord but did not enter this structure, as shown. Scale bars: 10 μm (A–D); 20 μm (E–G).