ZFIN Image: Bu et al., 2014, Fig. 6

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Fig. 6

Overexpressing gata1, zbp-89 or dominant-negative STAT3 rescues erythroid development in ptpn9a-morphant embryos and PTPN9-knockdown K562 cells. (A-C,G-L) βe1-globin was partially rescued in ptpn9a morphants (MO) that had been co-injected with gata1 mRNA (C), zbp-89 mRNA (I) or dominant-negative STAT3 (STAT3DN) mRNA (L), compared with wild-type (WT) controls (A,G,J) at 48 hpf. In addition, overexpression of gata1 mRNA rescued primitive erythrocytes in ptpn9a morphants (F), compared with wild-type controls (D), as revealed by o-Dianisidine staining. The numbers in the bottom left corner represent phenotypic embryos/total embryos. (M-P) Overexpression of STAT3DN rescued benzidine-positive erythroids (M) and γ-globin (N), CD235a (O) and CD71 (P) expression in K562 cells that had been infected with either mutant PTPN9 (N9CS) or transfected with PTPN9 shRNA (shN9) after induction with hemin for 48h, compared with vector (Vec) or scrambled (Scr) controls. The data represent the means of three independent experiments±s.d., *P<0.05.

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Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ J. Cell Sci.