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Fig. 8
Septin 7 forms a complex with Rab8. (A–C) Immunofluorescence staining of mIMCD3 cells shows partial colocalization of septin 7 (A) and Rab8 (B) in the perinuclear region, as shown by the yellow coloring in the merged image (C). Scale bar: 10μm. (D) Two different septin 7 antibodies (C or H-120) co-immunoprecipitate Rab8 in mIMCD3 cell lysates, indicating that septin 7 and Rab8 physically interact. IP, immunoprecipitation; IB, immunoblotting.
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Full text @ J. Cell Sci.