ZFIN Image: van der Vaart et al., 2014, Fig. 4

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Figure Caption

Fig. 4

DRAM1 Colocalizes with Mtb and Is Regulated by MYD88-NF-κB Signaling in Human Primary Macrophages

(A–C) The effect of NAI treatment on expression of (A) DRAM1, (B) SQSTM1, and (C) IL1B in M1 and M2 in the presence or absence of Mtb was determined by qPCR, relative to uninfected controls. All graphs show data (mean ± SEM) from three biological replicates.

(D) Expression of DRAM1 in M1 and M2 transfected with siCTRL or siMYD88 with and without Mtb infection. All graphs show data (mean ± SEM) from three biological replicates.

(E and F) Immunohistochemistry for (E) DRAM1 (green) or (F) LC3 (green) performed on M1 and M2 at 72 hpi with Mtb (red); Hoechst staining (blue) was used to outline the nuclei and cell boundaries. See also Figure S4.

Acknowledgments

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