ZFIN Image: Okigawa et al., 2014, Fig. S5

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Figure Caption

Fig. S5

Notch1a is required for the V2a/V2b cell fate determination. (A, B) V2a cell numbers were increased, whereas V2b cell numbers were slightly reduced in notch1a^th35b mutant embryos. V2a and V2b cells were detected by vsx2 (A) and sclα (B), respectively (75%, vsx2, n=12; 100%, sclα, n=11). All panels: side views of embryos at 24 hpf with anterior to the left, dorsal up. Dorsal and ventral borders of the neural tubes are shown by the dotted lines. Bar scale: 50 µm. Fig. S6: Effects of compound and single knockdown of Notch1b and Notch3 on V2 interneuron development. (A-E) V2a and V2b cells were identified as vsx1:GFP/Vsx2 double positive and vsx1:GFP/Scl double positive cells, respectively. Side views of embryos at 24 hpf with anterior to the left, dorsal up. Bar scale: 50 µm.

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Reprinted from Developmental Biology, 391(2), Okigawa, S., Mizoguchi, T., Okano, M., Tanaka, H., Isoda, M., Jiang, Y.J., Suster, M., Higashijima, S.I., Kawakami, K., Itoh, M., Different combinations of Notch ligands and receptors regulate V2 interneuron progenitor proliferation and V2a/V2b cell fate determination, 196-206, Copyright (2014) with permission from Elsevier. Full text @ Dev. Biol.