ZFIN Image: Dickover et al., 2014, Fig. 3

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Figure Caption

Fig. 3 rhoua knockdown does not affect cardiac cell fate specification and patterning. In situ analysis of AV and chamber specific cardiac genes revealed that cardiac cell fate specification and patterning were not perturbed in rhoua ATG MO injected zebrafish embryos. (A, B) vmhc and (C, D) amhc expression were restricted to the ventricular and atrial chambers, respectively, in (A, C) control and (B, D) rhoua MO1 knockdown embryos. (E, F) bmp4, (G, H) tbx2b, and (I, J) notch1b AV canal markers appeared to be expressed more broadly in the AV canal of (F, H, J) rhoua MO1 injected embryos when compared to (E, G, I) control MO injected embryos. (K, L) Conversely, anf was expressed in the chambers but not at the AV canal in (K) control and (L) rhoua MO injected embryos. Ventral view of heart. Anterior is to the top. Dashed lines outline the heart. Arrowhead points to AV canal. At – atrium, V – ventricle.

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Acknowledgments

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Reprinted from Developmental Biology, 389, Dickover, M., Hegarty, J.M., Ly, K., Lopez, D., Yang, H., Zhang, R., Tedeschi, N., Hsiai, T.K., Chi, N.C., The atypical Rho GTPase, RhoU, regulates cell-adhesion molecules during cardiac morphogenesis, 182-91, Copyright (2014) with permission from Elsevier. Full text @ Dev. Biol.