|
Fig. 5 Activities of zWnt4b is regulated by zNoxa. (a) Expression of zWnt4b in embryos, which were WT (i) and injected with zNoxa MO1+2 (ii), zNoxa mRNA (iii), zNoxa MO1+2 plus zNoxa rcRNA (iv), mNoxa mRNA (v), together mNoxa mRNA with zNoxa mRNA (vi), zMcl1a&b mRNA (vii), or zMcl1a&b MO (viii), was detected by in situ hybridization. (b) Expression of zWnt4b in embryos for each group in a. (c) Expression pattern of zWnt4b was detected by in situ hybridization using specific antisense or sense probes. No staining was present in embryos hybridized with sense probe (the shield stage shown). Embryo were lateral views with anterior toward the top. (d) Expression of zWnt4b in embryonic development stages was checked by real-time PCR and normalized to β-actin mRNA. Error bars represent S.E.M. (n=3). (e) Zebrafish embryos were collected at different stages and protein was extracted for immunoblot analysis of zWnt4b and β-actin after discarding the yolk. β-actin served as a loading control