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Fig. S1
The PTB-MO blocks Prdm12b translation and disrupts eng1b expression. (A) Expression of eng1b and krox20 in 24 hpf wild type embryo. (B) Morpholino-mediated knockdown of Prdm12b translation. In vitro translation reactions were resolved by PAGE, Western blotted and biotinylated Prdm12b was detected using avidin-alkaline phosphatase (bottom panel). Ponseau S staining (top panel) was used to demonstrate even loading of samples. (C, D) eng1b Expression in 50 hpf wild type (C) and PTB MO-injected (D) embryos. All embryos are in dorsal views with anterior to the left. Scale bar is 10 μm.
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Reprinted from Developmental Biology, 390, Zannino, D.A., Downes, G.B., Sagerström, C.G., prdm12b specifies the p1 progenitor domain and reveals a role for V1 interneurons in swim movements, 247-60, Copyright (2014) with permission from Elsevier. Full text @ Dev. Biol.