ZFIN Image: Jetti et al., 2014, Fig. 3

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Figure Caption

Fig. 3

Spontaneous Activity in the Dorsal Habenula Is Structured into Functionally, Spatially, and Genetically Distinct Microcircuits that Govern Odor Responses

(A and B) Spontaneous (A) and food-odor-evoked (B) activity of dHb neurons (y axis) are clustered using k-means clustering (left). Arrows indicate food-odor delivery. Spatial positions of dHb neurons in each cluster are marked with the corresponding cluster color (right). Scale bars represent 50 μm.

(C) A significant portion of dHb neurons remained within the same functional cluster during spontaneous and odor-evoked activity (n = 11). This is higher than chance (rank-sum test, p < 0.0005).

(D) Pairwise correlation of spontaneous and odor-evoked activity versus distance between dHb neurons.

(E) elavl3:GCaMP5;GAL4^s1019t/UAS:mCherry zebrafish dHb (top). The GAL4^s1019t/UAS:mCherry neurons (red) are identified among other (green) dHb neurons (middle). Functional clusters in the dHb based on k-means clustering are shown (bottom). Note that the red neurons overlap with the cyan and blue functional clusters.

(F and G) GAL4^s1019t neurons (red) are represented by significantly fewer clusters (F) and have more correlated spontaneous and odor-evoked activity (G) than the remaining green neurons (n = 6; properties of red and green neurons in each fish are represented by one green and red circle connected by a line). Paired t test, p < 0.05, p < 0.005.