Fig. S7

Fig. S7

Upregulation of Notch results in a broader domain of sox2 expression, at 20 ss. (A-D′′) sox2 is expressed throughout the pineal anlage and is downregulated in isl1-positive cells, in heat-shocked control embryos, at 20 ss. (A-D) sox2 expression, (A′-D′) isl1 expression, (A′′-D′′) merged images of sox2, isl1 and myc-tag, showing that heat-shock did not activate the Notch1a intracellular domain. (E-H′′) Heat-shock of the double transgenics Tg(hs:Gal4); Tg(UAS:Notch-intra) results in fewer isl1-positive cells (E′-H′) and therefore sox2 is expressed in a broader domain (E-H). (E′′-H′′) Merged images of sox2, isl1 and myc-tag, showing the activation of the transgene and thus the upregulation of Notch. Series of optical sections from dorsal (first column) to ventral (4^th column) obtained using confocal microscope, scale bars = 25 μm, yellow arrows show cells in which sox2 is downregulated and isl1 is upregulated, insets show a three times magnified view of the image. See also Figure 5, Movie S2.