ZFIN Image: Pavlou et al., 2014, Fig. S5

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Figure Caption

Fig. S5

Downregulation of sox2 does not affect Notch activity within the pineal gland. (A–C) Tg(csl:venus) is a Notch reporter line and drives venus expression (B) within the pineal gland, which is marked by isl1 antibody staining (A). Only about 50% of venus-expressing cells are positive for isl1. (D–F) Microinjections with sox2 morpholinos have no effect on Notch activity, as shown by venus expression. (G) Average number of total venus-positive cells (venus⁺/isl1⁺ and venus⁺/isl) in control (purple bars) and sox2 morphants (orange bars) at 24 and 28 hpf. Confocal maximum projections, scale bars = 25 µm, error bars represent ± standard error, MWU test; not significant; p-value >0.05, number of embryos counted is shown in each bar. See also Figure 5, Figure S6–S8 and Movie S1–S2.

Acknowledgments

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