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Fig. S4
sox2 controls the PhR cell fate. (A–D) aanat2 is expressed in the photoreceptors between 24 and 48 hpf, as detected by whole mount in situ hybridization. (E–H) At all stages analyzed, an upregulation of aanat2 expression is observed in sox2 morphants when compared to control siblings. (I–K) pax6b is expressed in the projection neurons and a subset of pineal precursors from 28 hpf to 48 hpf. (L–N) Downregulation of sox2 does not affect the number of pax6b-positive cells. At 48 hpf, pax6b is expressed in a broader domain in sox2 morphants when compared to control siblings. Developmental stages are shown at the bottom of each column, scale bars = 25 µm. See also Figure 4.