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Fig. 5
Wnt/β-Catenin Signaling Regulates Many Other Signaling Pathways
(A) Eigengene values of three correlated WGCNA modules, representing the average relative expression values of all genes in the module, in ten experimental conditions. Values shown are the average of triplicate samples relative to 0 hpa wild-type samples. Error bars indicate error of the mean.
(B) Cartoon of genes found in module 26 with genes annotated to be associated with Wnt, Fgf, and Hh signaling highlighted.
(C) Samples in (D)–(M) were either heat shocked at 42 hpa (blue) or 66 hpa (green) and harvested 6 hr later.
(D–M) Inhibition of Wnt/β-catenin signaling via axin1 or dkk1 overexpression interferes with pathway activity or expression of ligands, pathway components, and target genes of Hh (D), Bmp (E), RA (F), Igf (G), Notch (I and J), and Fgf (K) signaling. Expression of msxB and actβAa but not actβB remains unaffected using the same heat shock protocol (L and M).
(H) axin1 overexpression for 6 hr reduces Igf1r phosphorylation as detected in western blots of whole regenerates at 72 hpa.
(I) axin1 overexpression interferes with Notch signaling-dependent her4.3 promoter activity in her4.3:TetA AmCyan; hs:Axin1 double transgenic fish.
In (D)–(M), scale bars, 200 μm.