ZFIN Image: Wehner et al., 2014, Fig. 2

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Figure Caption

Fig. 2

β-Catenin Pathway Activity Is Detected in Different Blastemal Compartments with Distinct Proliferative Properties

(A) gfp transcripts (green) in 6xTCF:dGFP transgenic regenerates are strongly detected in cells of the distal blastema and in cells medially to the Runx2⁺ osteoblast progenitors (red; asterisk). gfp transcripts are only weakly detected in Runx2⁺ preosteoblasts (arrowheads).

(B) aldh1a2 (green) and mCherry RNA (red) are coexpressed in the distal-most blastema in 7xTCF:mCherry transgenic regenerates.

(C) gfp RNA in 6xTCF:dGFP regenerates is detected in the distal-most, Pcna-negative blastema (arrow) and in a subpopulation of the Pcna⁺, proximal blastema (arrowheads).

(D) gfp is coexpressed with and1 in the actinotrichia forming cells of 6xTCF:dGFP transgenics. Asterisk: (pre)osteoblasts. Note that the absence of Wnt reporter activity in the (pre)osteoblasts is due to a less sensitive staining procedure.

In (A)–(D), confocal images of longitudinal sections (A and C–D) or whole mounts (B). Scale bars, 100 μm and 10 μm (whole mount) and 100 μm (sections).

Acknowledgments

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