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Fig. S5
Splice-blocking MO against Nogo-C2 and NgRH1a also disrupted PLL development in zebrafish. Zebrafish embryos were injected with splice-blocking MOs against either (A) Nogo-C2 or (B) NgRH1a, and the neuromasts were stained with 4-Di-2-ASP at 72 hpf (upper left panels). The numbers of PLL neuromasts in these morphants at 72 hpf are summarized (lower left panels). The MO dosages used and sample numbers (N) are indicated. The efficiency and specificity of Nogo-C2-SB-MO and NgRH1a-SB-MO were confirmed by RT-PCR with two primer sets, as illustrated in the top right panels. The sequences of splice-blocking MOs and primers used in RT-PCR are provided.