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Fig. S4
Control experiments for morpholino specificity. To confirm the specificities of the MOs against three Nogo ligands and four Nogo receptors, pCMV-GFP reporter plasmids containing a perfect or mismatched MO target sequence corresponding to that MO were generated. The pCMV-GFP reporter plasmids bearing the perfect MO target sequence (mo-GFP) were injected into zebrafish embryos either alone (A–G) or together with the relevant MO (A2–G2). As controls, zebrafish embryos were co-injected with MO and pCMV-GFP reporter plasmid containing the mismatched target sequence (MM mo-GFP) (A3–G3). All images were taken from zebrafish embryos at 48 hpf.