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Fig. S3
ATG-MOs reduced the protein levels of p75 and TROY, and splice-blocking MOs also affected PLL development. (A–B) Total proteins from (A) p75 and (B) TROY morphant embryos were collected at 24 hpf and subjected to Western blotting with anti-p75 (abcam, ab32888) or anti-TROY (Enzo, ALX-210-801) antibodies, as indicated. GADPH was used as a loading control. (C–D) Zebrafish embryos were injected with splice-blocking MOs against either (C) p75 or (D) TROY, and the neuromasts were stained with 4-Di-2-ASP at 72 hpf (upper left panels). The numbers of PLL neuromasts in these morphants at 72 hpf are summarized (lower left panels). The MO dosages used and sample numbers (N) are indicated. The efficiency and specificity of p75-SB-MO and TROY-SB-MO were verified by RT-PCR with two primer sets, as illustrated in the top right panels. The sequences of splice-blocking MOs and primers used in RT-PCR are provided.