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Fig. S7 Acsl4a does not significantly alter Smad2/3 signaling, related to Figure 7. (A) Whole-mount mRNA in situ hybridization of ntl in 50% epiboly stage embryos, uninjected (control) or injected with 500 fmol acsl4a MO. Animal pole view. 96% of acsl4a MO-injected embryos appear wild-type (n=3 experiments; 127 embryos). (B) Sequence alignment of zebrafish Smad1/5 and Smad2/3 linker regions with important residues highlighted.
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Reprinted from Developmental Cell, 27(6), Miyares, R.L., Stein, C., Renisch, B., Anderson, J.L., Hammerschmidt, M., and Farber, S.A., Long-Chain Acyl-CoA Synthetase 4A Regulates Smad Activity and Dorsoventral Patterning in the Zebrafish Embryo, 635-647, Copyright (2013) with permission from Elsevier. Full text @ Dev. Cell