ZFIN Image: Cox et al., 2014, Fig. S4

Image

Figure Caption

Fig. S4

GSNOR inhibition is hepatoprotective and enhances survival in embryos exposed to APAP via activation of the Nrf2 pathway.

(A) Effect of chemical modulators of NO signaling during APAP-induced liver injury in Tg(lfabp:GFP) embryos. Tg(lfabp:GFP) embryos were exposed to a sublethal dose of APAP (5 mM) from 48 to 96 hpf in the presence or absence of either Deta (10 μM) or L-NAME (10 μM). Representative fluorescent photomicrographs were taken at 10x magnification. (B) Survival in embryos exposed to a lethal dose of APAP (10 mM) from 48 to 96 hpf in the presence or absence of the alternate NO donors Deta (10 μM) or GSNO (5 μM). N=75; Fisher’s exact test, *p<0.001. (C and D) Effect of GSNORi (1 μM) and/or APAP (5 mM) exposure from 48-72 hpf on induction of the Nrf2 target genes, prdx1 and hmox1, as determined by in situ hybridization at 72 hpf. Translation blocking ATG morpholino against nrf2 was injected at a concentration of 50 μM.

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Cell Rep.