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Fig. 2 Microinjection of EGFPSuDN perturbs arterial differentiation. (A,B) In situ hybridization of ephrinB2 in injected embryos fixed at 24 hpf. (A) Embryos injected with 800 pg of EGFP express ephrinB2 in the DA (red arrowhead). (B) In embryos injected with 800 pg of EGFPSuDN there is reduction of vascular ephrinB2. (C,D) In situ hybridization of notch3 in injected embryos fixed at 24 hpf. (C) Embryos injected with 1 ng of EGFP express notch3 within the neural tube (black arrow) and DA (red arrowhead). (D) notch3 expression within the neural tube (black arrow) and the DA (red arrowhead) is reduced following injection of 1 ng of EGFPSuDN. (E,F) In situ hybridization of grl in injected embryos fixed at 24 hpf. (E) Embyros injected with 800 pg EGFP express grl within the DA; (F) grl expression is not affected by injection with 800 pg EGFPSuDN. (A-F) All embryos were injected with indicated amount of mRNA at the 1-cell stage. (G, H) Transmitted light images of living embryos at 36 hpf. (G) Wild-type embryo injected with 800 pg of EGFP mRNA. (H) Wild-type embryo injected with 800 pg of EGFPSuDN exhibiting trunk curvature. (A-F) Lateral views, anterior to the left. (A-F) Scale bars (A-F) 100 μm, (G, H) 200 μm.