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Fig. S1
Injection of full-length wildtype nlrc3-like mRNA can fully rescue microglia in nlrc3-like–/– mutants, Related to Figure 1.
(A) Images of embryos injected with synthetic nlrc3-like mRNA at the 1-4 cell stage and uninjected controls. At 4–5 dpf, neutral red staining showed that introduction of synthetic nlrc3-like mRNA can fully (e 30 microglia) or partially (6–29 microglia) rescue the nlrc3-like mutants; there was no change in microglia formation in injected heterozygous or wildtype siblings. Dorsal views; anterior to the top. (B) Graph showing frequency of the different classes of microglia number (0–5, 6–29, or e 30 microglia) in the indicated genotypes in control (uninjected or water injected) and nlrc3-like mRNA injected groups. Number below bar graph represents n, number of embryos analyzed.
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