ZFIN Image: Wang et al., 2014, Fig. S9

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Figure Caption

Fig. S9

Notch3 is insufficient to induce ectopic brain pericyte specification and forced Notch3ICD expression in the endothelium failed to rescue the pericyte deficit in notch3^fh332 larvae. (A,B) Heat shock of Tg(hsp70I:Notch3ICD-EGFP) embryos at 30 hpf did not result in increase of brain pericyte number at 72 hpf. (A) Non-transgenic control larvae. (B) Tg(hsp70I:N3ICDEGFP) larva heat shocked at 30 hpf. (C,D) Transient N3ICD overexpression partially rescued the brain pericyte deficit phenotype in notch3^fh332 mutant larvae at 72 hpf. (C) notch3^fh332 mutant mock-injected control. (D) notch3^fh332 mutant injected with hsp70I:N3ICDGFP construct and heatshocked at 48 hpf. (E,F) Transient N3ICD expression in endothelial cells failed to rescue the brain pericyte deficit phenotype in notch3^fh332 mutant larvae. (E) notch3^fh332 mutant mock-injected control. (F) notch3^fh332 mutant injected with fli1:myc-Notch3ICD construct and heat shocked at 48 hpf. Arrowheads, pdgfrb+ brain pericytes. Scale bar, 200 μm.

Acknowledgments

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