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Fig. 1 klf17 expression in the EVL shortly after MBT depends on Pou5f1. Analysis of klf17 expression at embryonic stages from 1000 cells to 75% epiboly in WT (A–E), Mspg (F–H) and MZspg embryos (I–M). All embryos are shown in animal (left column) or lateral view (right column); shield and 75% epiboly stages are oriented with dorsal to the right. Insets show lateral regions of transversal sections of embryos. (N) Microarray data for klf17 expression in WT, Mspg and MZspg embryos at sphere stage; rpl5b is not regulated by Pou5f1 and was used as normalization control.
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Reprinted from Developmental Biology, 385(2), Kotkamp, K., Mössner, R., Allen, A., Onichtchouk, D., and Driever, W., A Pou5f1/Oct4 dependent Klf2a, Klf2b, and Klf17 regulatory sub-network contributes to EVL and ectoderm development during zebrafish embryogenesis, 433-47, Copyright (2014) with permission from Elsevier. Full text @ Dev. Biol.