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Fig. 5 col2a1, shh, and Engrailed expression in mutants in the sly, gup, bal, dop, sny, and gno loci. Shown are DIC micrographs of (A-C) wildtype embryos, and (D-F) slym466, (G-I) gupm189, (J-L) balm190, (M-O) dopm341, (P-R) snym456, and (S-U) gnom622 mutant embryos stained by in situ hybridization with anti-sense zebrafish col2a1 (A,D,G,J,M,P,S), shh (B,E,H,K,N,Q,T) RNA or with monoclonal anti-Engrailed antibody 4D9 (C,F,I,L,O,R,U). Embryos stained for col2a1 are at 32 hpf and embryos stained for shh or Engrailed are at 24 hpf. Scale bars, 250 μm.
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