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Fig. 10 Rescue of the horizontal myoseptum phenotype in ubo and yot mutants by cell transplantation. Rhodamine dextran-labeled wildtype cells were transplanted into ubo or yot mutants at mid-late blastula stage. Embryos were scored for rescue of the myoseptum at 48-72 hpf. (A) Normarski picture of five segments with a rescued myoseptum in an ubo mutant. Note that the segment in the middle does not show any rescue. (B) Picture of the same embryo showing the fluorescently labeled wild type cells. In all segments with rescue, labeled cells are present in the myoseptum. In the myotome that did not show rescue only muscle fibers, that are slightly above or far below the myoseptum, are labeled. (C) Normarski picture of a yot mutant with a single rescued horizontal myoseptum (arrow). (D) Picture of the same region showing a brightly labeled wild-type cell at the position where the myoseptum formed.