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Fig. S8 Injection of Emilin3 cDNA rescues the phenotype of Emilin3 morphants. (A) Lateral views of 24 hpf embryos co-injected with the indicated morpholinos (4 ng/embryo) and with the indicated cDNA constructs (25 ng/μl). Arrows point at notochord distortions in Emilin3 double morphants, which are largely rescued by co-injection with the pCS-twhh-mEmilin3 construct. The fraction of embryos displaying the corresponding phenotype is provided in each panel. (B) Whole mount immunofluorescence labeling for engrailed and Emilin3 in 24 hpf embryos injected as in (A). Scale bar, 25 μm. (C) Quantification of engrailed-positive cells in 24 hpf co-injected embryos (*, P < 0.05; n.s., not significant; n = 10). Ctrl MO, control morpholino; e3a+e3b MO, emilin3a + emilin3b morpholinos; Trunk MPs, trunk muscle pioneers; Trunk MFFs, trunk medial fast fibers.