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Fig. S7 Hedgehog upregulation in Emilin3 morphant embryos is not dependent on BMP or TGF-β signaling. (A) Lateral views of 24 hpf embryos injected with the indicated morpholinos and treated at 8 hpf with 5 μM cyclopamine (lower panels) or with the corresponding volume of ethanol as a control (upper panels). Embryos were then stained with the monoclonal 4D9 antibody (green) and the polyclonal pSMAD1/5/8 antibody (purple). The panels show magnifications around somite 10. Scale bar, 50 μm. (B) Wild-type embryos were injected with the indicated morpholinos. Embryos were then left untreated or treated at 8 hpf with 30 μM of the selective TGF-β receptor inhibitor LY364947 from 8 hpf and probed for ptc1 expression at 24 hpf. The fraction of embryos displaying the corresponding phenotype is provided in each panel. Ctrl MO, control morpholino; e3a+e3b MO, emilin3a + emilin3b morpholinos.