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Fig. S5 Hedgehog upregulation in Emilin3 morphant embryos is dependent on Emilin3 protein depletion. (A, B) Embryos were injected with the indicated splice- or translation-blocking morpholinos (ATG) and probed for ptc1 expression at 24 hpf. Lateral view of the trunk, head is on the left. (C) Control and Emilin3 double morphant embryos were probed for ptc1 expression at 8-somite stage. (D) Dorsal view of wild-type embryos immunostained for Emilin3 and β-catenin at at 8-somite stage. Note the absence of Emilin3 in the notochord (marked by the asterisks) at this stage of development. Scale bar, 100 μm. The fraction of embryos displaying the corresponding phenotype is provided in each panel. Ctrl MO, control morpholino; e3a+e3b MO, emilin3a + emilin3b morpholinos; p53 MO, p53 morpholino.