|
Fig. S4 Analysis of collagen II and laminin expression in Emilin3 morphant embryos. (A, B) Zebrafish embryos were injected with the indicated morpholinos and probed for col2a1 expression at 24 and 48 hpf, respectively. The fraction of embryos displaying the corresponding phenotype is provided in each panel. Note that at 24 hpf, even embryos injected with emilin3a or emilin3b morpholino alone displayed a slight increase in col2a1 expression. (C) Lateral views of controls and Emilin3 morphant embryos labeled by immunohistochemistry for laminin at 24 hpf. The panels display projection of confocal sections around somite 10, showing the notochord. Scale bar, 25 μm. Ctrl MO, control morpholino; e3a MO, emilin3a morpholino; e3b MO, emilin3b morpholino; e3a+e3b MO, emilin3a + emilin3b morpholinos.