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Fig. 4 Expression of crh in otpa and otpb single and double mutant larvae.
Whole-mount in situ hybridization of 3 dpf larvae reveals loss of crh expression in the preoptic region and posterior tuberculum of otpa;otpb double mutant larvae (arrowhead and asterisk, respectively). Dorsal view, anterior at left. Scale bar is 50 μm. Abbreviations: H, hypothalamus; PO, preoptic region; PT, posterior tuberculum.
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