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Fig. 3 Vangl2 functions in floor plate cells in r4 to regulate FBM neuron migration. Panels A,D show dorsal views of 36 hpf (A) and 48 hpf (D) Tg(isl1:GFP) host hindbrains, and panels B,E show cross-sections at the levels indicated by the broken lines. Vangl2 mutants are indicated as tri/ throughout. (A,B) Presence of vangl2 mutant donor cells in the floor plate (fp) in r4 (arrows) of a wildtype host can abrogate (partially block) the migration of some host FBM neurons (black arrowheads) out of r4. A large number of host neurons (white arrowhead) migrate caudally. (C) Quantification of vangl2 mutant>Wt transplantation data. Number of embryos in parentheses. (D,E) Floor plate-specific expression of vangl2 (arrows) in a vangl2 mutant completely rescues the migration of host FBM neurons (arrowhead). Gal4+ve floor plate cells are green, Myc-Vangl2+ve cells are red or yellow. Asterisks indicate Gal4FF-induced GFP expression in otic vesicle, trunk muscles, and skin. Many GFP-expressing cells are Myc-Vangl2ve (and vice versa) probably due to independent and variable silencing of the UAS:GFP and UAS:Myc-vangl2 transgenes integrated at different sites. (F) Quantification of Gal4-UAS rescue data. Number of embryos in parentheses. fp, floor plate.
Reprinted from Developmental Biology, 382(2), Sittaramane, V., Pan, X., Glasco, D.M., Huang, P., Gurung, S., Bock, A., Li, S., Wang, H., Kawakami, K., Matise, M.P., and Chandrasekhar, A., The PCP protein Vangl2 regulates migration of hindbrain motor neurons by acting in floor plate cells, and independently of cilia function, 400-412, Copyright (2013) with permission from Elsevier. Full text @ Dev. Biol.