ZFIN Image: Sittaramane et al., 2013, Fig. S8

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Figure Caption

Fig. S8 Expression of vangl2 transgene in floor plate cells can rescue FBM neuron migration in vangl2 (tri-/-) mutants.
(A,D) Dorsal views of the hindbrain of tri-/-; Tg(Fp:Gal4FF; UAS:GFP); Tg(UAS:Myc-vangl2); Tg(isl1:GFP) embryos immunostained with anti-MYC antibody (red) and anti-GFP antibody (green). Presence of Myc-Vangl2-expressing floor plate cells in r4 (arrows) effectively rescues the migration of vangl2 mutant FBM neurons (A and D, black arrowheads). (B,C) Cross sections of the embryo in A at the indicated levels showing Myc-Vangl2 expression in the floor plate (B, arrow) and migrated vangl2 mutant FBM neurons in r6 (C, black arrowhead). (E,F) Cross sections of the embryo in D at the indicated levels showing Myc-Vangl2 expression in the floor plate (E, arrow) and migrated vangl2 mutant FBM neurons in r6 (F, black arrowhead). Non-migrated neurons (white arrowheads) are seen in D and E. Floor plate cells are yellow due to GFP expression from the UAS:GFP transgene. Asterisks indicate Gal4FF-driven expression of the UAS:GFP transgene in otic vesicle and skin. The Gal4FF driver does not induce UAS:GFP expression in branchiomotor neurons. oto, otic vesicle; fp, floor plate.

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Reprinted from Developmental Biology, 382(2), Sittaramane, V., Pan, X., Glasco, D.M., Huang, P., Gurung, S., Bock, A., Li, S., Wang, H., Kawakami, K., Matise, M.P., and Chandrasekhar, A., The PCP protein Vangl2 regulates migration of hindbrain motor neurons by acting in floor plate cells, and independently of cilia function, 400-412, Copyright (2013) with permission from Elsevier. Full text @ Dev. Biol.