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Fig. 1 Knockdown of vangl2 and prickle1a decreases fibronectin. (A–C) Top panels show morphological convergence and extension phenotypes at tailbud stage (see arrowheads) of wild type, vangl2 morphant, and prickle1a morphant embryos. Middle/bottom panels show confocal images of fibronectin (Fn) immunolabeling without and with nuclear DAPI staining. Scale bars=20 μm. (D and E) Fn and GAPDH western blots of 10 μg total protein extract obtained from tailbud stage wild type, vangl2 mutant, glypican4 mutant, and prickle1a morphant embryos.
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Reprinted from Developmental Biology, 383(1), Dohn, M.R., Mundell, N.A., Sawyer, L.M., Dunlap, J.A., and Jessen, J.R., Planar cell polarity proteins differentially regulate extracellular matrix organization and assembly during zebrafish gastrulation, 39-51, Copyright (2013) with permission from Elsevier. Full text @ Dev. Biol.