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Fig. S1 tal1-deficient embryos contain a normal number of endocardial cells. Frontal views of wild-type (A and B) and tal1-deficient hearts (C and D) at 30 hpf; ventricular end of the heart tube at the top. The transgene Tg(fli1a:negfp) (green) was used to count the number of endocardial cells residing within the boundaries of the myocardium, which was labeled by MF20 immunofluorescence (red).
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Reprinted from Developmental Biology, 383(2), Schumacher, J.A., Bloomekatz, J., Garavito-Aguilar, Z.V., and Yelon, D., tal1 regulates the formation of intercellular junctions and the maintenance of identity in the endocardium, 214-226, Copyright (2013) with permission from Elsevier. Full text @ Dev. Biol.