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Fig. 1 Filopodia facilitate anastomosis of DLAV and the vein plexus. (A,B) Endothelial tip cells of untreated (A) and 0.08 μg/ml Lat. B-treated (B) 31 hpf Tg(Fli1ep:Lifeact-EGFP); Tg(Kdr-l:ras-Cherry)s916 zebrafish embryos at the dorsal roof of the neural tube. Arrows indicate polymerisation of F-actin at new cell contacts. Time is in minutes. (C-D′) Vein plexi of Tg(Fli1ep:Lifeact-EGFP); Tg(Kdr-l:ras-Cherry)s916 embryos treated with DMSO or 0.1 μg/ml Lat. B from 31 to 46 hpf. Arrowheads indicate junctional F-actin. DA, dorsal aorta; DV, dorsal vein; VV, ventral vein. Scale bars: 10 μm.
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