ZFIN Image: Colombo et al., 2013, Fig. S6

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Figure Caption

Fig. S6 Local electroporation of C-DAAM1 in the right habenular region induces increased axonal efferent connectivity to the IPN, without affecting habenular neuropil. (A-F) Dorsal views of maximum intensity z-stack confocal projections of the habenular region (A-D) and IPN (E,F) in Tg(pou4f1-hsp70l:GFP) embryos at 4.5 dpf, with anterior to the top. The habenular neuropil was immunostained against acetylated α-tubulin (A,B, white), while the soma (C,D) and efferent projections (E,F) of dorsal habenular neurons expressing pou4f1-hsp70l:GFP were detected in vivo (green). Each column corresponds to a different condition of local electroporation: control plasmid (left) and pcDNA-C-DAAM1-HA (right). Asterisks in C and D indicate the enlarged cellular domain of the Hb expressing the pou4f1-hsp70l:GFP transgene. Scale bar, 20μm.

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