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Fig. S6 In vivo functional evaluation of nek2 and rpgr deficiency in zebrafish. (A) Ocular phenotypes are quantified in control, nek2 MO, rpgr MO, and nek2 MO + rpgr MO embryos at full and sub-effective doses. (B) Immunohistochemical analyses of retinal cryosections from control, nek2 MO, rpgr MO, and nek2 + rpgr MO embryos at sub- effective doses, stained with DAPI (blue) and 4D2 antibody (green). Asterisk denotes excessive rhodopsin in the outer nuclear layer (ONL). OS, outer segment of photoreceptors; INL, inner nuclear layer.
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