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Fig. 3
Injection of wnt4 MO affects HC formation in zebrafish embryos. Tg(foxd3:GFP) embryos were used to inject control (A–C) and wnt4a (D–F) MO, fixed at 54 hpf and stained with anti-GFP (green) (A and D) and anti-acetylated tubulin (red) (B and E). C and F are merged images. D–F are rotated counterclockwise by about 20°. G: Dose-dependency of the MO effect. Experiments were compared to control HC, and statistical significance and number of embryos are indicated as specified in the legend to Figure 1.
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