ZFIN Image: Andersson et al., 2012, Fig. 2

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Figure Caption

Fig. 2

The Hit Compounds Increase Regeneration, Not Survival, of β Cells

(A) Schematic diagram for cell labeling and assessment of β cell survival/regeneration. To examine β cell survival, we made use of the photoconvertible property of the fluorescent protein Kaede. At 3 dpf, before ablating the β cells with MTZ from 3-4 dpf, we converted Tg(ins:Kaede)-expressing β cells from green to red by exposing them to UV light. After 2 days of regeneration (6 dpf), the surviving β cells are red and green (yellow overlap), whereas the newly formed β cells are green only.

(B and C) Confocal images of DMSO- and NECA-treated larvae with Tg(ins:Kaede)-expressing β cells at 6 dpf. Note that there is one β cell that survived the ablation in this particular DMSO-treated larva (arrow in B), whereas there are no β cells that survived the ablation in this NECA-treated larva (C).

(D) Quantification of β cell regeneration (green bars) and β cell survival (yellow bars) per larva at 6 dpf, following treatment with DMSO, NECA, Cilostamide, or A-134974 from 4-6 dpf. p < 0.0001; n = 10 larvae for each group. Error bars represent SEM. See also Figure S2.

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Expression / Labeling details

Phenotype details

Acknowledgments

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Reprinted from Cell Metabolism, 15(6), Andersson, O., Adams, B.A., Yoo, D., Ellis, G.C., Gut, P., Anderson, R.M., German, M.S., and Stainier, D.Y., Adenosine Signaling Promotes Regeneration of Pancreatic beta Cells In Vivo, 885-894, Copyright (2012) with permission from Elsevier. Full text @ Cell Metab.