ZFIN Image: Burkhalter et al., 2013, Fig. S4

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Figure Caption

Fig. S4

Rapamycin Rescues the Lateralization Phenotype also during Ciliogenesis Stages and upon Ablation of Grk5l in the KV, Related to Figure 4

(A) Rapamycin treatment from tailbud stage (10 hpf) rescues the Grk5l phenotype similarly to the treatment during gastrulation. Same day clutches of NI, CTRL MO and Grk5l MO injected embryos were treated with rapamycin from 10 hpf on. Bar graph of spaw misexpression. CTRL MO^DMSO versus Grk5l MO^DMSO: p < 0.0001; Grk5l MO^DMSO versus Grk5l MO^Rapamycin: p = 0.0152; Fisher’s exact test.

(B) Coimmunoprecipitation of endogenous GRK5 with endogenous Raptor from HEK293T cells. Asterisks indicate unspecific background staining in the rabbit IgG control, which originates from crossreaction with the secondary anti-rabbit antibody. IP, immunoprecipitation; WCL, whole cell lysate.

(C) Randomized spaw expression caused by KV-specific depletion of Grk5l can be normalized by rapamycin treatment from 6 to 10 hpf. CTRL MO^DMSO versus Grk5l MO^DMSO: p < 0.0001; Grk5l MO^DMSO versus Grk5l MO^Rapamycin: p = 0.0017; Fisher’s exact test.

Acknowledgments

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