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Fig. S3
Design and validation of tmem88a morpholinos. (A) Morpholino design. A translation blocker (tbMO) was targeted to the translation start site (atg) of tmem88a mRNA. A splice-site blocker (ssMO) was targeted to the boundary between intron 2 (ii) and exon 3 (3) of the mRNA. (B) The efficacy of the ssMO was monitored by semi-quantitative reverse-transcription PCR. The abundance of the normal tmem88a transcript (196 bp) was reduced with optimal doses of ssMO. An unspliced transcript (2890 bp) accumulated that is predicted to encode a premature stop codon (UGA), confirmed by sequencing. Levels of β-actin were used as a loading control.