ZFIN Image: Novikov et al., 2013, Fig. 8

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Figure Caption

Fig. 8

Loss of CPs in tmem88a morphants is not caused by increased apoptosis, decreased cell proliferation or fate conversion to the hematovascular or forelimb lineages. Dorsal views of representative flatmount preparations of 8-somite (A-H) and 23-somite (I,J) control and tmem88a morphants, with anterior to the top (n>20 embryos analyzed per condition). (A,B) WISH for nkx2.5 and ntl, showing the cardiogenic mesoderm (arrowheads). (C,D) Apoptosis detection by TMR-Red. (E,F) Phospho-Histone H3 antibody staining for cell proliferation. (G,H) WISH for the hematovascular marker scl and for ntl. Arrow indicates the boundary of the anterior scl expression domain. (I,J) WISH for the forelimb marker tbx5.

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Acknowledgments

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