Fig. 3
tmem88a transcript localizes to cardiac progenitors. (A,B) Dorsal views (anterior to the left) of flatmount in situ hybridization of wild-type 8-somite stage zebrafish embryos stained for nkx2.5/ntl and tmem88a/ntl. Insets are higher magnifications of the boxed regions. (C,D) Laterals views of whole-mount in situ hybridization (WISH) preparations of wild-type 14-somite stage embryos stained for nkx2.5/ntl and tmem88a/ntl. Insets are higher magnification dorsal views of the boxed regions. (E,F) Dorsal views of cardiogenic mesoderm (anterior to the left) in flatmount dual fluorescent in situ hybridization preparations of wild-type embryos stained for nkx2.5/ntl or tmem88a at the 8- or 14-somite stage. Arrowheads indicate CPs. n>20 embryos analyzed per panel.